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name: wb-troubleshooter description: Western Blot troubleshooting guide with step-by-step solutions version: 1.0.0 category: Wet Lab tags: [] author: AIPOCH license: MIT status: Draft risk_level: Medium skill_type: Tool/Script owner: AIPOCH reviewer: '' last_updated: '2026-02-06'

WB Troubleshooter

Western Blot problem diagnosis and solutions.

Use Cases

• High background troubleshooting
• No signal diagnosis
• Multiple bands resolution
• Band smearing fixes

Parameters

• symptom: Problem description
• antibody_type: Primary/Secondary

Returns

• Systematic troubleshooting steps
• Likely causes ranked
• Solution recommendations

Example

Input: "High background" Output: Check blocking buffer → antibody concentration → wash stringency

Risk Assessment

Risk Indicator	Assessment	Level
Code Execution	Python/R scripts executed locally	Medium
Network Access	No external API calls	Low
File System Access	Read input files, write output files	Medium
Instruction Tampering	Standard prompt guidelines	Low
Data Exposure	Output files saved to workspace	Low

Security Checklist

• No hardcoded credentials or API keys
• No unauthorized file system access (../)
• Output does not expose sensitive information
• Prompt injection protections in place
• Input file paths validated (no ../ traversal)
• Output directory restricted to workspace
• Script execution in sandboxed environment
• Error messages sanitized (no stack traces exposed)
• Dependencies audited

Prerequisites

No additional Python packages required.

Evaluation Criteria

Success Metrics

• Successfully executes main functionality
• Output meets quality standards
• Handles edge cases gracefully
• Performance is acceptable

Test Cases

1. Basic Functionality: Standard input → Expected output
2. Edge Case: Invalid input → Graceful error handling
3. Performance: Large dataset → Acceptable processing time

Lifecycle Status

• Current Stage: Draft
• Next Review Date: 2026-03-06
• Known Issues: None
• Planned Improvements:
  • Performance optimization
  • Additional feature support